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KMID : 0894520070110030205
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Development & Reproduction
2007 Volume.11 No. 3 p.205 ~ p.217
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Development of a Primary Tissue Culture Method having Greater Reliability than Isolated Cell Cultures - Steroid-Responsiveness of Uterine Myometrial and Myomatous(Leiomyomatous) Cells
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Lee Eun-Ju
Prati Bajracharya
Hyun Jin-Hee
Kim Hang-Jin
Song Gun-Ho
Cho Kyung-Hyun
Lee Dong-Mok
Lee Taek-Hoo
Chun Sang-Sik
Choi In-Ho
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Abstract
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As an initial step toward better understanding of the molecular mechanism of estrogen-dependent growth in myoma, an optimal primary cell culture condition has been developed and examined by this study. Myoma and myometrium were cultured by two different methods. Culture stability and E2-responsiveness in stable culture were studied. The culture of digested tissue pieces(Method 2) was found to be a stable culture method for the myoma and myometrium showing a favorable response to estrogen. mRNA expression of PR, IGF-1 and IGF-1 receptor genes was enhanced by E2. The gene responses to E₂ were higher in myoma compared with myometrium. Moreover, these responses were more expressive in tissues than in the surrounding cells in primary culture of normal myometrium and myoma, implying a vital role of cell communication through the extracellular matrix in maintaining the estrogen-responsiveness. The development of an improved cell culture system for myoma provides an in vitro tool to further investigate the basis of the tumor formation.
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KEYWORD
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Cell culture, Estrogen, Myoma(Leiomyoma or fibroid), Uterine tumor
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